Fertilization and Egg Collection Process

one: Classic IVF Method(In Vitro Fertilization = IVF )

It is generally preferred for couples with young, high number of eggs and good sperm characteristics. The follicle fluid, which is sent to the embryo laboratory in sterile tubes, is transferred to a special medium, which is examined under a microscope by embryologists in a special cabinet. Eggs and sperms obtained from the sick couple are left together in special containers prepared for this procedure (approximately 50 thousand to 100 thousand motile sperm are placed for each egg) and kept for 16-18 hours, during which time the sperm will fertilize the egg by itself. After this process, fertilization control is done.

2: microinjection( Intra Cytoplasmic Sperm Injection = ICSI )

In cases where sperm count and motility are low, deformity is high, and sperm retrieval by surgical method (TESA, TESE). Microinjection is preferred in cases where the number of eggs taken from the woman is insufficient, the quality is low, the egg membrane is thick, in cases of unexplained infertility and in couples who have failed IVF before. The eggs collected from the patient are purified from the remnants of the surrounding cells (cumulus cells) after the sorting process, and the mature ones are selected for the process. The fertilization process is done 2-6 hours after the eggs are taken from the patient.

Sperm are injected into the egg with a needle by means of a special device called a micromanipulator under the microscope by embryologists. incubatorFertilization control is done after it is kept for 16-18 hours (in a special cabinet that provides an environment similar to the mother’s womb).

Today, ICSI, that is, microinjection, is preferred instead of IVF as in vitro fertilization treatment. The reason for this is the increase in the fertilization rate in the microinjection process. Since a single sperm cell is sufficient for the normal fertilization of an egg, even men with low sperm count can be allowed to have children with the microinjection method.

OPU ; Egg Collection Process; It is the process of emptying the fluid-filled structures called follicles that contain eggs.

The final stage of egg development cracking needle36 hours after the injection to the patient, two hours before the procedure to be applied, the patient was called and asked his wife. sperm A sample is taken and preliminary information is given about the process. Since egg collection will be performed, the patient should not eat or drink anything from 24:00 at night. This is a procedure that takes 15-20 minutes under general anesthesia in the operating room.

The patient, who is taken to the operating room, is placed on the gynecological table and the ovaries are accessed with a special needle adapted to the transvaginal ultrasonography probe. By entering each follicle, the follicle fluid is discharged into the tubes with the help of an aspirator. The emptied follicles are examined by embryologists and it is reported whether there are eggs in them. If the egg is not obtained, the follicle cavity is washed with a special liquid and any eggs that may have remained in it are removed. In this way, the process is continued until all follicles are aspirated. After collecting all the eggs over 10 mm, the egg collection process is terminated.

The eggs obtained are separated from the incoming liquid, collected in another container containing culture liquid, their number is determined and stored in special cabinets that provide an environment similar to the mother’s womb until fertilization. Eggs collected may differ from the number determined by ultrasound during follow-up. What matters at this stage is how many eggs we have. After the procedure, the patient is awakened and taken to his room to rest.

If all controls are normal after the rest and the patient feels well, then the drug recipes to be used are explained, information is given about the eggs collected and the patient is discharged. There may be mild pain that may occur after this procedure and bleeding in the form of leaks or drops that may continue for several days.

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